MOLECULAR CLONING AND CHARACTERIZATION OF A SERINE PROTEINASE GENE OF TRICHINELLA SPIRALIS

2003 ◽  
Vol 89 (1) ◽  
pp. 92-98 ◽  
Author(s):  
Isao Nagano ◽  
Zhiliang Wu ◽  
Takumi Nakada ◽  
Thidarut Boonmars ◽  
Yuzo Takahashi
Keyword(s):  
Molecular Cloning ◽  
Trichinella Spiralis ◽  
Serine Proteinase

scholarly journals P106 Molecular cloning and characterization of enolase gene from Trichinella spiralis

2001 ◽  
Vol 52 (Supplement) ◽  
pp. 104
Author(s):  
Takumi NAKADA ◽  
Isao NAGANO ◽  
Zhiliang WU ◽  
Yuzo TAKAHASHI
Keyword(s):  
Molecular Cloning ◽  
Trichinella Spiralis

scholarly journals P107 Molecular cloning and characterization of serine protease of Trichinella spiralis

2001 ◽  
Vol 52 (Supplement) ◽  
pp. 104
Author(s):  
Yuzo TAKAHASHI ◽  
Isao NAGANO ◽  
Zhiliang WU ◽  
Takumi NAKADA
Keyword(s):  
Molecular Cloning ◽  
Serine Protease ◽  
Trichinella Spiralis

Trichinella spiralisnewborn larvae: characterization of a stage specific serine proteinase expression, NBL1, using monoclonal antibodies

Parasitology ◽  
2015 ◽  
Vol 142 (6) ◽  
pp. 783-790 ◽  
Author(s):  
YONG YANG ◽  
SANDRINE A. LACOUR ◽  
VÉRONIQUE LAINÉ-PRADE ◽  
NICOLAS VERSILLÉ ◽  
AURÉLIE GRASSET-CHEVILLOT ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Developmental Stages ◽  
Trichinella Spiralis ◽  
Serine Proteinase ◽  
Mammalian Skeletal Muscle ◽  
Serological Tests ◽  
Common Motif ◽  
Major Antigen ◽  
Gravid Females

SUMMARYTrichinella spiralisis an intracellular parasitic nematode of mammalian skeletal muscle, causing a serious zoonotic disease in humans and showing a high economic impact mainly in pig breeding. Serine proteinases ofT. spiralisplay important roles in the host–parasite interactions mediating host invasion. In this study, we have focused on newborn larvae (NBL-1), the first identified serine proteinase from the NBL stage ofT. spiralis.Five monoclonal antibodies (mAbs) directed against the C-terminal part of NBL1, were produced. These mAbs were IgG1κisotype and specifically recognized as a common motif of 10 amino acids (PSSGSRPTYP). Selected mAbs were further characterized using antigens from various developmental stages ofT. spiralis.Western blot revealed that selected mAbs reacted with the native NBL1 at Mr50 kDa in the adult and NBL mixed antigens and NBL stage alone. Indirect immunofluorescence analysis revealed that selected mAbs intensely stained only the embryos within the gravid females and the NBL. Thus, the produced mAbs are useful tools for the characterization of NBL1 as a major antigen ofTrichinellainvolved in the invasion of the host but also for the development of new serological tests with an early detection ofT. spiralisinfection.

scholarly journals Molecular Cloning, Expression, and Partial Characterization of Two Novel Members of the Ovalbumin Family of Serine Proteinase Inhibitors

1995 ◽  
Vol 270 (50) ◽  
pp. 29854-29861 ◽  
Author(s):  
Cindy A. Sprecher ◽  
Kurt A. Morgenstern ◽  
Shannon Mathewes ◽  
Jeffrey R. Dahlen ◽  
Sara K. Schrader ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Proteinase Inhibitors ◽  
Serine Proteinase ◽  
Partial Characterization ◽  
Serine Proteinase Inhibitors

Molecular cloning and characterization of heat shock protein 70 from Trichinella spiralis

Acta Tropica ◽  
2009 ◽  
Vol 110 (1) ◽  
pp. 46-51 ◽  
Author(s):  
Shaohua Wang ◽  
Xinping Zhu ◽  
Yaping Yang ◽  
Jing Yang ◽  
Yuan Gu ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Molecular Cloning ◽  
Heat Shock Protein 70 ◽  
Trichinella Spiralis
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